无论该薄层色谱法是否是药典方法，不满足使用需求时，展开剂比例均可以调节的。

欧洲药典2.2.46对薄层色谱法对允许调节的参数进行了具体说明，如下，供参考：

THIN-LAYER CHROMATOGRAPHY

Composition of the mobile phase: the amount of the minor solvent components may be adjusted by ± 30 per cent relative or ± 2 per cent absolute, whichever is the larger; no other component is altered by more than 10 per cent absolute. A minor component comprises less than or equal to (100/n) per
cent, n being the total number of components of the mobile phase. For a minor component at 10 per cent of themobile phase, a 30 per cent relative adjustment allows a range of 7-13 per cent whereas a 2 per cent absolute adjustment allows a range of 8-12 per cent, the relative value therefore being the larger ; for a minor component at 5 per cent of the mobile phase, a 30 per cent relative adjustment allows a range of 3.5-6.5 per cent whereas a 2 per cent absolute adjustment allows a range of 3-7 per cent, the absolute value being the larger in this case.

pH of the aqueous component of the mobile phase: ± 0.2 pH units, unless otherwise prescribed.
Concentration of salts in the buffer component of a mobile phase: ± 10 per cent.
Application volume: 10-20 per cent of the prescribed volume if using fine particle size plates (2-10 μm).

通常，这些参数在上述调节范围内，默认仍是药典分析方法的。

如展开剂调节超出了上述范围，需要补充研究证明满足使用目的的需求，通常至少对其Rf值、检出限值进行确认。

其他药典，展开剂调节后，类比做补充研究，同时注意满足对应市场药典的通则及色谱技术要求即可。